N-Acetyl-Cysteine Ethyl Ester

Description

N-Acetyl-Cysteine Ethyl Ester Overview

N-Acetyl-Cysteine Ethyl Ester (NACE or NACET) is a modified derivative of N-acetyl-L-cysteine (NAC) with a molecular weight of 163.2 g/mol. Through esterification, NACET demonstrates increased lipophilicity compared with NAC, which may enhance cellular permeability in experimental models.

Within laboratory environments, NACE is investigated for its role in cellular antioxidant systems and its relationship to cysteine and glutathione metabolism. Once internalized in experimental systems, NACET may undergo enzymatic de-esterification, generating NAC and subsequently cysteine, a precursor involved in the biosynthesis of glutathione (GSH).

Chemical and Molecular Properties

PubChem CID	148861
Molecular Formula	C7H13NO3S
Molecular Weight	191.25 g/mol
Synonyms	Ac-Cys-OEt (R)-ethyl 2-acetamido-3-mercaptopropanoate, Ethyl (2R)-2-acetamido-3-sulfanylpropanoate
IUPAC	ethyl (2R)-2-acetamido-3-sulfanylpropanoate
CAS	59587-09-6
Labeling	Research Use Only (RUO), not for human or animal consumption
Chemical Structure Depiction
Purity	99% Purity
Classification	Research Use Only (RUO), not for human or animal consumption
Storage Temperature	Store at -20°C (-4°F) for long-term stability
Solubility	Water, DMSO (research-grade solvents)
Safety	Handle with gloves, a lab coat, eye protection; use a fume hood if dust/aerosol is possible

Mechanisms of Action

The molecular mechanisms associated with NAC-derived compounds are complex and continue to be explored in biochemical and cellular research.

In experimental models, NAC-related compounds are known to interact with several metabolic and redox-regulated pathways. One of the primary mechanisms involves supplying cysteine, which contributes to intracellular glutathione synthesis.

Research observations suggest NAC-derived compounds may participate in:

• Cellular glutathione replenishment pathways
• Detoxification of certain reactive intermediates
• Redox-regulated cellular signaling processes
• Interactions with reactive oxygen species in controlled laboratory conditions

Potential Research Applications

N-Acetyl-Cysteine Ethyl Ester (NACE or NACET) is investigated in laboratory environments due to its relationship to cysteine metabolism and intracellular redox regulation. Its esterified structure may allow increased cellular permeability compared with non-esterified NAC in certain experimental systems.

Researchers study NACE in controlled experimental models to examine biochemical pathways associated with oxidative balance, glutathione synthesis, and cellular signaling.

Potential areas of investigation include:

• Cellular Redox Studies: Examination of oxidative stress responses and redox-regulated signaling pathways in cultured cells.
• Glutathione Pathway Research: Investigation of cysteine availability and its role in intracellular glutathione biosynthesis.
• Membrane Permeability Studies: Comparative analysis of cellular uptake and transport between NAC and esterified derivatives such as NACET.
• Mitochondrial and Metabolic Research: Evaluation of mitochondrial oxidative balance and energy-related cellular processes in experimental models.
• Sulfur Metabolism Research: Study of sulfur-containing intermediates and redox-active molecules involved in cellular signaling pathways.

Disclaimer

This information is for educational purposes only and not medical advice. Products are for research use only. Research must follow IRB or IACUC guidelines. Verify information independently before purchasing. By ordering, you agree to our Terms and Conditions. If you are not 100% satisfied with the product you received, please contact us at support@staging.purerawz.co

ATTENTION: All our products are for LABORATORY AND RESEARCH PURPOSES ONLY, not for veterinary or human use

References:

1. Arranz, L., Fernandez, C., Rodriguez, A., Ribera, J. M., & De La Fuente, M. (2008). The glutathione precursor N-acetylcysteine improves immune function in postmenopausal women. Free Radical Biology and Medicine, 45(9), 1252-1262. https://doi.org/10.1016/j.freeradbiomed.2008.07.014
2. Cocco, T., Sgobbo, P., Clemente, M., Lopriore, B., Grattagliano, I., Di Paola, M., & Villani, G. (2004). Tissue-specific changes of mitochondrial functions in aged rats: Effect of a long-term dietary treatment with N-acetylcysteine. Free Radical Biology and Medicine, 38(6), 796-805. https://doi.org/10.1016/j.freeradbiomed.2004.11.034
3. Pallotta, V., Gevi, F., D'Alessandro, A., & Zolla, L. (2014). Storing red blood cells with vitamin C and N-acetylcysteine prevents oxidative stress-related lesions: a metabolomics overview. www.ncbi.nlm.nih.gov. https://doi.org/10.2450/2014.0266-13