Jackhammer Tablets (Mirodenafil + Amino Tadalafil + SR-9011)

Description

Overview

This research stack is a multi-compound formulation used in investigational laboratory research to explore interactions between cyclic nucleotide signaling pathways and nuclear receptor-mediated transcriptional regulation. The stack contains Mirodenafil, Amino Tadalafil, and SR-9011, compounds that belong to different pharmacological classes frequently examined in in vitro studies and preclinical experimental models.

Two compounds in the stack, Mirodenafil and Amino Tadalafil, function as phosphodiesterase type-5 (PDE5) inhibitors, commonly utilized in research investigating cyclic guanosine monophosphate (cGMP) signaling pathways. The third compound, SR-9011, is a synthetic agonist of REV-ERB nuclear receptors, which are key transcriptional regulators involved in circadian rhythm control and metabolic gene expression.

Mirodenafil

Mirodenafil is a selective phosphodiesterase type-5 (PDE5) inhibitor studied in biochemical and molecular pharmacology research involving cyclic nucleotide signaling pathways. In investigational research settings, mirodenafil is frequently used to analyze mechanisms regulating intracellular cyclic guanosine monophosphate (cGMP) levels.

PDE5 inhibitors are widely utilized in in vitro enzyme inhibition studies and experimental models investigating nitric oxide-mediated signaling pathways and cyclic nucleotide-dependent cellular processes. Through selective interaction with PDE5, mirodenafil serves as a research tool for evaluating enzyme kinetics and signal transduction mechanisms related to cGMP metabolism.

Chemical and Molecular Properties

Property	Value
Compound Name	Mirodenafil
CAS Number	862189-95-5
Molecular Formula	C26H37N5O5S
Molecular Weight	531.7 g/mol
Chemical Class	Phosphodiesterase-5 inhibitor

Working Mechanism

Mirodenafil inhibits phosphodiesterase type-5 (PDE5), an enzyme responsible for the hydrolysis of cyclic guanosine monophosphate (cGMP).

Within cellular signaling systems:

1. Nitric oxide signaling activates guanylate cyclase, generating intracellular cGMP.
   
2. PDE5 normally degrades cGMP into inactive 5'-GMP.
   
3. Mirodenafil binds to the catalytic domain of PDE5, preventing enzymatic hydrolysis of cGMP.
   

In experimental biochemical systems, this results in sustained intracellular cGMP levels, which may be used to investigate:

• protein kinase G (PKG) activation pathways
  
• cyclic nucleotide-dependent signal transduction
  
• intracellular pathways associated with smooth-muscle cell signaling and vascular cell biology
  

These molecular interactions are typically studied using enzyme assays and cellular signaling experiments in laboratory environments.

Amino Tadalafil

Amino Tadalafil is a structural derivative related to tadalafil, investigated in experimental pharmacological research as a phosphodiesterase-5 inhibitory compound. Compounds within this class are widely used in in vitro biochemical research models to examine cyclic nucleotide metabolism and PDE enzyme selectivity.

In laboratory studies, tadalafil analogs provide a platform for exploring second messenger signaling systems, particularly those involving cGMP-mediated intracellular pathways.

Chemical and Molecular Properties

Property	Value
Compound Name	Amino Tadalafil
Molecular Formula	C21H18N4O4
Molecular Weight	390.4 g/mol
Chemical Class	PDE5 inhibitor analog

Working Mechanism

Amino Tadalafil derivatives function as competitive inhibitors of phosphodiesterase-5 in biochemical systems.

The molecular process involves:

1. Binding to the active site of PDE5.
   
2. Blocking enzymatic degradation of cyclic guanosine monophosphate (cGMP).
   
3. Sustaining intracellular levels of this cyclic nucleotide in experimental conditions.
   

Elevated cGMP concentrations within research models enable investigation of:

• PKG-dependent signaling cascades
  
• cyclic nucleotide-regulated transcriptional processes
  
• intracellular pathways associated with cellular signaling and metabolic regulation
  

These mechanisms are commonly examined using enzyme inhibition assays and cellular signaling experiments.

SR-9011

SR-9011 is a synthetic agonist of the REV-ERB nuclear receptors, specifically REV-ERBα and REV-ERBβ, which are ligand-regulated transcriptional repressors involved in the molecular circadian clock.

In investigational research, SR-9011 is frequently utilized in in vitro studies and preclinical experimental models to investigate circadian rhythm regulation, transcriptional repression mechanisms, and metabolic gene expression pathways.

Chemical and Molecular Properties

Property	Value
Compound Name	SR-9011
CAS Number	1379686-29-9
Molecular Formula	C23H31ClN4O3S
Molecular Weight	479.0 g/mol
Chemical Class	REV-ERB nuclear receptor agonist

Working Mechanism

SR-9011 acts as a ligand for REV-ERBα and REV-ERBβ nuclear receptors, which function as transcriptional repressors within the circadian regulatory network.

The mechanism involves:

1. Binding of SR-9011 to the ligand-binding domain of REV-ERB receptors.
   
2. Recruitment of corepressor complexes, including NCoR and HDAC3.
   
3. Suppression of transcription of REV-ERB target genes.
   

REV-ERB signaling interacts with the CLOCK-BMAL1 transcriptional system, which regulates circadian gene expression. Through this pathway, SR-9011 is used in research models investigating:

• circadian transcriptional repression
  
• metabolic gene regulation associated with circadian cycles
  
• interactions between nuclear receptors and transcriptional regulators
  

These processes are typically examined using cell culture experiments, gene expression profiling, and molecular reporter assays.

Research Applications in Laboratory Settings

Cyclic Nucleotide Signaling Research

Mirodenafil and amino tadalafil analogs are widely used in in vitro enzymatic assays to examine:

• PDE5 inhibition kinetics
  
• regulation of intracellular cGMP signaling pathways
  
• activation of PKG-dependent signaling cascades
  

These experiments often involve enzyme inhibition studies and cyclic nucleotide quantification assays.

Circadian Biology Research

SR-9011 is frequently used in experimental models investigating circadian transcriptional regulation.

Research applications include:

• analysis of REV-ERB-mediated gene repression
  
• evaluation of circadian clock transcription networks
  
• investigation of clock-controlled metabolic gene expression
  

Such studies typically employ cell culture models and transcriptional reporter systems.

Metabolic Gene Expression Studies

In preclinical molecular biology research, the interaction between REV-ERB signaling and cyclic nucleotide pathways may be evaluated using multi-compound experimental systems such as Jackhammer.

Investigations may focus on:

• transcriptional responses of metabolic regulatory genes
  
• interaction between circadian clock components and cellular metabolism
  
• regulatory networks linking energy metabolism and transcription factors

Multi-Pathway Signaling Research Models

The presence of compounds targeting distinct biological systems allows researchers to design integrated experimental models examining cross-talk between signaling pathways.

Potential research areas include:

• integration of second messenger signaling with nuclear receptor transcription pathways
  
• modulation of metabolic regulatory networks
  
• molecular interactions between circadian gene regulators and metabolic signaling cascades
  

These investigations are typically performed using controlled in vitro experiments, biochemical assays, and gene expression profiling techniques.

Why Choose Purerawz for Mirodenafil + Amino Tadalafil + SR-9011?

Buy Mirodenafil + Amino Tadalafil + SR-9011 for laboratory research use from our online shop. At Purerawz, we provide high-quality reference materials. Each research compound comes with a Certificate of Analysis for verification of purity and concentration.

Disclaimer

This information is for educational purposes only and not medical advice. Products are for research use only. Research must follow IRB or IACUC guidelines. Verify information independently before purchasing. By ordering, you agree to our Terms and Conditions. If you are not 100% satisfied with the product you received, please contact us at support@staging.purerawz.co

Reference Links

• Kim, F., Singh, P., Jo, H., Xi, T., Song, D., Ku, S. K., & Choung, J. J. (2024). Therapeutic effects of mirodenafil, a phosphodiesterase 5 inhibitor, on stroke models in rats. Neurotherapeutics, 22(1), e00463. https://doi.org/10.1016/j.neurot.2024.e00463
• Sadek, A. A., Sabra, M. S., Ali, M. F., Abdelhamid, H. N., & Hussein, K. (2025). Potential of tadalafil and tadalafil-cellulose nanocomposite in preventing postsurgical abdominal adhesions in a rat cecal abrasion model. Scientific Reports, 15(1), 31210. https://doi.org/10.1038/s41598-025-14894-0
• Sandu, C., Dumas, M., Malan, A., Sambakhe, D., Marteau, C., Nizard, C., Schnebert, S., Perrier, E., Challet, E., Pevet, P., & Felder-Schmittbuhl, M. (2012). Human skin keratinocytes, melanocytes, and fibroblasts contain distinct circadian clock machineries. Cellular and Molecular Life Sciences, 69(19), 3329-3339. https://doi.org/10.1007/s00018-012-1026-1